Application
Immunohistochemistry staining of MAPK1 in heart tissue using MAPK1 Monoclonal Antibody.| Product Name | MAPK1 Antibody [4C11C11C4] |
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| Antibody Type | Primary Antibodies |
| Product description | ERK2, a MAPK type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK2 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK2 causing dissociation of the MEK/ERK complex. A portion of the activated ERK2 pool forms dimers and translocates to the nucleus to activate nuclear proteins, such as ELK-1 and MSK1. Cytosolic ERK 2 acts on a different set of substrates, including MNK1/2 and MAPKAP-K1. |
| Immunogen | MAPK1 monoclonal antibody was raised against Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| MAPK1 antibody can be used in Western Blot starting at 1:500 - 1:3000, immunohistochemistry starting at 1:400, immunoprecipitation, and immunofluorescence. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK1 |
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| Alternative Names | MAPK1 ERK ERK-2 ERK2 MAP kinase 1 MAPK 2 MAPK2 MAP kinase isoform p42 MAPK 1 p41mapk PRKM2 MAP kinase 2 p41 p42-MAPK PRKM1 ERT1 p38 p40 p42MAPK Protein tyrosine kinase ERK2 |
Application
Immunohistochemistry staining of MAPK1 in heart tissue using MAPK1 Monoclonal Antibody.| Application Notes | MAPK1 antibody can be used in Western Blot starting at 1:500 - 1:3000, immunohistochemistry starting at 1:400, immunoprecipitation, and immunofluorescence. |
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| Form | Liquid |
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| Storage Instructions | Store MAPK1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.01% sodium azide. |
