Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Product Name | MARCH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH1 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase MARCH1 632- Membrane-associated RING finger protein 1 Membrane-associated RING-CH protein I MARCH-I RING finger protein 171 MARCH1 RNF171 |
| Molecular Weight(MW) | 32 kDa |
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
