Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Product Name | MARCH10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).1) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) |
| Immunogen | This MARCH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-274 amino acids from the Central region of human MARCH10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH10 |
|---|---|
| Alternative Names | Probable E3 ubiquitin-protein ligase MARCH10 632- Membrane-associated RING finger protein 10 Membrane-associated RING-CH protein X MARCH-X RING finger protein 190 MARCH10 RNF190 |
| Molecular Weight(MW) | 91 kDa |
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
