Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates thdetected the 40613 protein (arrow).| Product Name | MARCH11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al., 2007 [PubMed 17604280]).1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This MARCH11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-337 amino acids from the C-terminal region of human MARCH11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH11 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase MARCH11 632- Membrane-associated RING finger protein 11 Membrane-associated RING-CH protein XI MARCH-XI MARCH11 |
| Molecular Weight(MW) | 44 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates thdetected the 40613 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
