Application
Western blot analysis in T47D cell line lysates (35ug/lane).| Product Name | MARCH7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).1) Szigyarto, C.A., et al. J. Histochem. Cytochem. 58(4):301-308(2010) |
| Immunogen | This MARCH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human MARCH7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH7 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase MARCH7 632- Axotrophin Membrane-associated RING finger protein 7 Membrane-associated RING-CH protein VII MARCH-VII RING finger protein 177 MARCH7 AXOT RNF177 |
| Molecular Weight(MW) | 78 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in T47D cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
