Application
Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.Application
Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 μg/mL.| Product Name | MARCH8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.1) Coscoy L, Sanchez DJ, and Ganem D. A novel class of herpesvirus-encoded membrane-bound E3 ubiquitin ligases regulates endocytosis of proteins involved in immune recognition. J. Cell Biol. 2001; 155:126573. |
| Immunogen | MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8. The immunogen is located within the last 50 amino acids of MARCH8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH8 |
|---|---|
| Alternative Names | MARCH8 Antibody: MIR c-MIR RNF178 MARCH-VIII MIR E3 ubiquitin-protein ligase MARCH8 Cellular modulator of immune recognition |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Application
Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MARCH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MARCH8 Antibody is supplied in PBS containing 0.02% sodium azide. |
