Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human Muscle| Product Name | Rabbit anti-MARCH8 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCH-VIII, MIR, RNF178, c-MIR |
| Immunogen | The immunogen for anti-MARCH8 antibody: synthetic peptide directed towards the middle region of human MARCH8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH8 |
|---|---|
| Gene Synonyms | MARCH-VIII MIR RNF178 c-MIR |
| Gene Full Name | Membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase |
| Gene Summary | MARCH8 is an E3 ubiquitin-protein ligase that may regulate immune responses by promoting ubiquitination of MHC-II and CD86, which leads to their subsequent endocytosis and lysosomal degradation. It may also promote ubiquitination and endocytosis of TFRC and FAS. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Alternative Names | MARCH-VIII MIR RNF178 c-MIR |
| Molecular Weight(MW) | 33kDa |
| Sequence | 291 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human Muscle| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
