Application
Western blot analysis of MARCH8 (arrow) using rabbit polyclonal MARCH8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARCH8 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of anti-MARCH8 Antibody (Center) in K562 cell line lysates (35ug/lane). MARCH8 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARCH8 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MARCH8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MARCH8; MIR; RNF178; E3 ubiquitin-protein ligase MARCH8; Cellular modulator of immune recognition; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; RING finger protein 178 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIR RNF178 |
|---|---|
| Alternative Names | MARCH8 MIR RNF178 E3 ubiquitin-protein ligase MARCH8 Cellular modulator of immune recognition Membrane-associated RING finger protein 8 Membrane-associated RING-CH protein VIII RING finger protein 178 |
| Molecular Weight(MW) | 32965 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS |
| Tissue Specificity | Broadly expressed. Present in immature dendritic cells (at protein level) |
| Cellular Localization | Cytoplasmic vesicle membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein Early endosome membrane; Multi-pass membrane protein |
Application
Western blot analysis of MARCH8 (arrow) using rabbit polyclonal MARCH8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARCH8 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of anti-MARCH8 Antibody (Center) in K562 cell line lysates (35ug/lane). MARCH8 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARCH8 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
