Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Product Name | MARH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARCH3 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH3 appears to function in the endosomal recycling pathway (Fukuda et al., 2006 [PubMed 16428329]).1) Fukuda, H., et al. J. Biochem. 139(1):137-145(2006) |
| Immunogen | This MARH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human MARH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCH3 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase MARCH3 632- Membrane-associated RING finger protein 3 Membrane-associated RING-CH protein III MARCH-III RING finger protein 173 MARCH3 RNF173 |
| Molecular Weight(MW) | 29 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
