Application
Western blot analysis of MARK4 using rabbit polyclonal hMARK4-R626 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2).Application
Western blot analysis of anti-MARK4 Pab in mouse liver tissue lysate (35ug/lane).| Product Name | MARK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MARK4 contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1-associated kinase domain. RT-PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6-kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony-forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells. |
| Immunogen | This MARK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 611-641 amino acids from the C-terminal region of human MARK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MARK4 |
|---|---|
| Alternative Names | MAP/microtubule affinity-regulating kinase 4 MAP/microtubule affinity-regulating kinase-like 1 MARK4 KIAA1860 MARKL1 |
| Molecular Weight(MW) | 83 kDa |
Application
Western blot analysis of MARK4 using rabbit polyclonal hMARK4-R626 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2).
Application
Western blot analysis of anti-MARK4 Pab in mouse liver tissue lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
