Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of MASTL Antibody in HepG2, MDA-MB231, 293, Hela cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | MASTL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MASTL, microtubule associated serine/threonine kinase-like, contains 1 protein kinase domain which belongs to the Ser/Thr protein kinase family. It may be involved in megakaryocyte differentiation. Defects in MASTL are a cause of nonsyndromic autosomal1) Gandhi, M.J., et al., Hum. Hered. 55(1):66-70 (2003). |
| Immunogen | This MASTL antibody is generated from rabbits immunized with human partial MASTL recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MASTL |
|---|---|
| Alternative Names | Serine/threonine-protein kinase greatwall GW GWL hGWL Microtubule-associated serine/threonine-protein kinase-like MAST-L MASTL GW GWL THC2 |
| Molecular Weight(MW) | 97 kDa |
Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of MASTL Antibody in HepG2, MDA-MB231, 293, Hela cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
