WB
Western blot analysis of GWL on different lysates with Rabbit anti-GWL antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: LNCaP cell lysate, Lane 3: HepG2 cell lysate, Lane 4: MDA-MB-231 cell lysate, Lane 5: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling GWL with Rabbit anti-GWL antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GWL antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/50 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
GWL was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-GWL antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-GWL antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-GWL antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-GWL antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds.| Product Name | GWL Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human GWL aa 1-350. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:2000 ICC/IF:1:50 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MASTL |
|---|---|
| Gene Synonyms | GW GWL THC2 MAST-L GREATWALL |
| Gene Full Name | microtubule associated serine/threonine kinase like |
| Gene Summary | This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010] |
| Alternative Names | 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L 2700091H24Rik C88295 FLJ14813 GREATWALL Greatwall protein kinase GW GWL GWL_HUMAN hGWL MAST-L Mastl MGC117975 Microtubule associated serine/threonine kinase like Microtubule-associated serine/threonine-protein kinase-like RP11 85G18.2 Serine/threonine-protein kinase greatwall THC2 |
| Molecular Weight(MW) | 97kDa(Observed band size: 110kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus, Cleavage furrow. |
WB
Western blot analysis of GWL on different lysates with Rabbit anti-GWL antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: LNCaP cell lysate, Lane 3: HepG2 cell lysate, Lane 4: MDA-MB-231 cell lysate, Lane 5: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling GWL with Rabbit anti-GWL antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GWL antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/50 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
GWL was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-GWL antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-GWL antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-GWL antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-GWL antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds.| Application Notes | WB:1:2000 ICC/IF:1:50 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
