Application
Antibody used in WB on Human HepG2 at 0.625 ug/ml.Application
Antibody used in WB on Mouse ES at: 1:1000 (Lane 1: 15ug WT mouse ES lysate, Lane 2: 15ug MBD2 KO mouse ES lysate).| Product Name | MBD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.1) Le (2006) Mol. Cell. Biol. 26 (3), 843-851. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MBD2 antibody can be used for detection of MBD2 by ELISA at 1:1562500. MBD2 antibody can be used for detection of MBD2 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MBD2 |
|---|---|
| Alternative Names | MBD2 DMTase NY-CO-41 |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human HepG2 at 0.625 ug/ml.
Application
Antibody used in WB on Mouse ES at: 1:1000 (Lane 1: 15ug WT mouse ES lysate, Lane 2: 15ug MBD2 KO mouse ES lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | MBD2 antibody can be used for detection of MBD2 by ELISA at 1:1562500. MBD2 antibody can be used for detection of MBD2 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MBD2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
