Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).| Product Name | MBD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.1) Arora, M., et al. Leukemia 24(8):1470-1475(2010) |
| Immunogen | This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MBD4 |
|---|---|
| Alternative Names | Methyl-CpG-binding domain protein 4 322- Methyl-CpG-binding endonuclease 1 Methyl-CpG-binding protein MBD4 Mismatch-specific DNA N-glycosylase MBD4 MED1 |
| Molecular Weight(MW) | 66 kDa |
Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
