Application
MBOAT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Western blot analysis of MBOAT4 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse stomach tissue lysateApplication
Western Blot at 1:2000 dilution + mouse stomach lysate Lysates/proteins at 20 ug per lane.| Product Name | MBOAT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MBOAT4 mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.1) Takahashi, T., et al. J. Biochem. 146(5):675-682(2009) Gomez, R., et al. Arthritis Rheum. 60(6):1704-1709(2009) Gutierrez, J.A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(17):6320-6325(2008) |
| Immunogen | This MBOAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the Central region of human MBOAT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MBOAT4 |
|---|---|
| Alternative Names | Ghrelin O-acyltransferase 231- Membrane-bound O-acyltransferase domain-containing protein 4 O-acyltransferase domain-containing protein 4 MBOAT4 GOAT OACT4 |
| Molecular Weight(MW) | 50 kDa |
Application
MBOAT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Western blot analysis of MBOAT4 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse stomach tissue lysate
Application
Western Blot at 1:2000 dilution + mouse stomach lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
