Application
Immunohistochemistry of MC4R in human brain tissue with MC4R antibody at 2.5 μg/mL.Application
Immunofluorescence of MC4R in Human Brain cells with MC4R antibody at 20 μg/mL.Application
Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | MC4R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MC4R Antibody: The melanocortin-4 receptor (MC4R) is a member of the superfamily of seven transmembrane G-protein coupled receptors that are involved in multiple signal transduction pathways including the cAMP and MAPK signaling pathways. It is thought that the melanocortin system modulates energy expenditure and insulin sensitivity; activation of the MC4R results in the inhibition of c-Jun N-terminal kinase (JNK) activity and promotes insulin signaling. MC4R-null mice display maturity onset obesity characterized by hyperphagia, increased adiposity, hyperinsulinaemia and hyperleptinaemia, suggesting that like other obesity-linked genes such as FTO, PTER, and NPC1, MC4R is a potential candidate target for the treatment of obesity.1) Gantz I, Miwa H, Konda Y, et al. Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J. Biol. Chem.1993; 268:15174-9. |
| Immunogen | MC4R antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MC4R. The immunogen is located within the first 50 amino acids of MC4R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MC4R antibody can be used for detection of MC4R by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MC4R |
|---|---|
| Alternative Names | MC4R Antibody: Melanocortin receptor 4 MC4-R |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of MC4R in human brain tissue with MC4R antibody at 2.5 μg/mL.
Application
Immunofluorescence of MC4R in Human Brain cells with MC4R antibody at 20 μg/mL.
Application
Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | MC4R antibody can be used for detection of MC4R by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MC4R antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MC4R Antibody is supplied in PBS containing 0.02% sodium azide. |
