Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Intestine.| Product Name | MCM8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCM8 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.1) Clarke, C.A. Biochem. J. 388 (PT 2), 705-712 (2005). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| MCM8 antibody can be used for detection of MCM8 by ELISA at 1:1562500. MCM8 antibody can be used for detection of MCM8 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCM8 |
|---|---|
| Alternative Names | MCM8 C20orf154 MGC119522 MGC119523 MGC12866 MGC4816 REC dJ967N21.5 |
| Molecular Weight(MW) | 94 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Intestine.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | MCM8 antibody can be used for detection of MCM8 by ELISA at 1:1562500. MCM8 antibody can be used for detection of MCM8 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
