Application
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine MCP-2 (38-118) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.| Product Name | MCP-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-2 (murine MCP-2). Murine MCP-2 specific antibody was purified by affinity chromatography employing immobilized mMCP-2 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect mMCP-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2. Sandwich To detect mMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MCP-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2. Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Ccl8 |
|---|---|
| Alternative Names | HC14 Mcp2 MCP-2 Scya8 AB023418 1810063B20Rik C-C motif chemokine 8 Monocyte chemoattractant protein 2 |
Application
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine MCP-2 (38-118) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.| Application Notes | ELISA: Indirect: To detect mMCP-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2. Sandwich To detect mMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MCP-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2. Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MCP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
