| Product Name | MCP-2 Antibody [1.1_2D4-1A3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. |
| Immunogen | The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL8 |
|---|---|
| Alternative Names | CCL 8 HC14 Monocyte Chemotactic Protein 2 SCYA10 SCYA8 MCP2 C-C motif chemokine 8 MCP-2 |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
