| Product Name | MCP-4 Antibody [G7_89.3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MCP4 is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP-3, MCP-4 elicits chemo-attraction of monocytes and T lymphocytes |
| Immunogen | The monoclonal antibody was produced in BALB/c mice using recombinant human MCP-4 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rMCP-4 (100 ng/mL), a concentration of 1-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-4 when used with our biotinylated antigen affinity purified anti-human MCP-4 (XP-5225Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCL13 |
|---|---|
| Alternative Names | macrophage monocyte chemotactic protein 4 MCP4 NCC1 SCYA13 C-C motif chemokine 13 CK-beta-10 MCP-4 CKb10 NCC-1 SCYL1 |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rMCP-4 (100 ng/mL), a concentration of 1-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-4 when used with our biotinylated antigen affinity purified anti-human MCP-4 (XP-5225Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MCP-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
