| Product Name | MDA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. MDA5, upon interaction with intracellular dsRNA (produced during viral replication), triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappaB, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). MDA5 is widely expressed at low levels. The expression is detected at higher levels in placenta, pancreas and spleen and barely in brain, testis and lung. Genetic variation in MDA5 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. |
| Immunogen | Recombinant mouse MDA5 (aa 2-208). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Immunocytochemistry: (1:100). Functional Application: Blocks the RNA-dependent RNA polymerase activity in vitro. Note: Not suitable for Western blot. Use anti-NS5B (HCV) mAb (5B-3B1) (Prod. No. AG-20B-0002). | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Ifih1 |
|---|---|
| Alternative Names | Helicard Melanoma Differentiation-associated Gene 5 |
| Tissue Specificity | Recognizes mouse MDA5. |
| Entrez Gene | 71586 |
|---|---|
| Protein Accession | NP_082111.1 |
| Application Notes | Immunocytochemistry: (1:100). Functional Application: Blocks the RNA-dependent RNA polymerase activity in vitro. Note: Not suitable for Western blot. Use anti-NS5B (HCV) mAb (5B-3B1) (Prod. No. AG-20B-0002). |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. Neat serum containing 0.02% sodium azide. |
