Application
Immunohistochemistry of ME1 in mouse liver tissue with ME1 antibody at 5 μg/mL.Application
Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at 1 μg/ml.| Product Name | ME1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).1) Loeber G, Dworkin MB, Infante A, et al. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994; 344:181-6. |
| Immunogen | ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1. The immunogen is located within the first 50 amino acids of ME1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ME1 |
|---|---|
| Alternative Names | ME1 Antibody: MES HUMNDME NADP-dependent malic enzyme Malic enzyme 1 NADP-ME |
| Molecular Weight(MW) | Predicted: 63 kDa Observed: 63 kDa |
| Tissue Specificity | ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2. |
Application
Immunohistochemistry of ME1 in mouse liver tissue with ME1 antibody at 5 μg/mL.
Application
Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ME1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ME1 Antibody is supplied in PBS containing 0.02% sodium azide. |
