WB
Western blot analysis of ME1 using anti-ME1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ME1 polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ME1 using anti-ME1 antibody. ME1 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of ME1 using anti-ME1 antibody and anti-Beta Tubulin antibody. ME1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IF-P
IF analysis of ME1 using anti-ME1 antibody. ME1 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A549 cells using anti-ME1 antibody. Overlay histogram showing A549 cells stained with anti-ME1 antibody (Blue line). To facilitate intrME1llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ME1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ME1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ME1 recombinant protein (Position: M1-Q572). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ME1 |
|---|---|
| Gene Synonyms | MES HUMNDME |
| Gene Full Name | malic enzyme 1 |
| Gene Summary | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008] |
| Alternative Names | HUMNDME Malic enzyme 1 ME1 MES NADP dependent malic enzyme NADP ME |
| Molecular Weight(MW) | 64kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ME1 using anti-ME1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ME1 polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ME1 using anti-ME1 antibody. ME1 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of ME1 using anti-ME1 antibody and anti-Beta Tubulin antibody. ME1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IF-P
IF analysis of ME1 using anti-ME1 antibody. ME1 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-ME1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A549 cells using anti-ME1 antibody. Overlay histogram showing A549 cells stained with anti-ME1 antibody (Blue line). To facilitate intrME1llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ME1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
