Application
This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.Application
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hMEC by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.| Product Name | MEC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect hMEC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC. Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CCL28 |
|---|---|
| Alternative Names | MEC CCK1 SCYA28 C-C motif chemokine 28 Mucosae-associated epithelial chemokine MEC |
Application
This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Application
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hMEC by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.| Application Notes | ELISA: Sandwich: To detect hMEC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MEC (38-279) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC. Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
