Application
Western blot analysis of MECR in (A) human brain tissue, (B) mouse brain tissue, and (C) rat brain tissue lysate with MECR antibody at 1 μg/mL.Application
Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MECR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.1) Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33. |
| Immunogen | Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR. The immunogen is located within amino acids 300 - 350 of MECR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MECR antibody can be used for detection of MECR by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MECR |
|---|---|
| Alternative Names | MECR Antibody: NRBF1 CGI-63 FASN2B NBRF1 Trans-2-enoyl-CoA reductase mitochondrial Nuclear receptor-binding factor 1 HsNrbf-1 |
| Molecular Weight(MW) | Predicted: 41 kDa |
| Sequence Similarities | 3) Cat. No. 1463 - Rat Brain Tissue Lysate |
| Tissue Specificity | At least two isoforms of MECR are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of MECR in (A) human brain tissue, (B) mouse brain tissue, and (C) rat brain tissue lysate with MECR antibody at 1 μg/mL.
Application
Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | MECR antibody can be used for detection of MECR by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MECR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MECR Antibody is supplied in PBS containing 0.02% sodium azide. |
