Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | MED17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.1) Matsuoka, S., et al. Science 316(5828):1160-1166(2007) |
| Immunogen | This MED17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 332-361 amino acids from the Central region of human MED17. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED17 |
|---|---|
| Alternative Names | Mediator of RNA polymerase II transcription subunit 17 Activator-recruited cofactor 77 kDa component ARC77 Cofactor required for Sp1 transcriptional activation subunit 6 CRSP complex subunit 6 Mediator complex subunit 17 Thyroid hormone receptor-asso |
| Molecular Weight(MW) | 73 kDa |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
