Application
MED25 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates thdetected the MED25 protein (arrow).Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).| Product Name | MED25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].1) Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010)Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004) |
| Immunogen | This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED25 |
|---|---|
| Alternative Names | Mediator of RNA polymerase II transcription subunit 25 Activator interaction domain-containing protein 1 Activator-recruited cofactor 92 kDa component ARC92 Mediator complex subunit 25 p78 MED25 ACID1 ARC92 PTOV2 |
| Molecular Weight(MW) | 78 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
MED25 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates thdetected the MED25 protein (arrow).
Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
