Application
Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/mL.Application
Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 μg/mL.| Product Name | MED4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.1) Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91. |
| Immunogen | MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4. The immunogen is located within the last 50 amino acids of MED4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED4 |
|---|---|
| Alternative Names | MED4 Antibody: ARC36 VDRIP DRIP36 TRAP36 HSPC126 ARC36 Mediator of RNA polymerase II transcription subunit 4 Activator-recruited cofactor 36 kDa component |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform. |
Application
Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/mL.
Application
Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MED4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MED4 Antibody is supplied in PBS containing 0.02% sodium azide. |
