Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | MED8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.1) Leonard, V.H., et al. J. Virol. 80(19):9667-9675(2006) |
| Immunogen | This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-122 amino acids from the Central region of human MED8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED8 |
|---|---|
| Alternative Names | Mediator of RNA polymerase II transcription subunit 8 Activator-recruited cofactor 32 kDa component ARC32 Mediator complex subunit 8 MED8 |
| Molecular Weight(MW) | 29 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Zebrafish |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
