Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).Application
Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).| Product Name | MEK 2 (phospho Thr394) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.1) Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852. |
| Immunogen | MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2. |
| Modification | p-Thr394 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K2 |
|---|---|
| Alternative Names | CFC4 MEK2 MKK2 MAPKK2 PRKMK2 SRC1 ERK activator kinase 2 MAP kinase kinase 2 MAP2K2 MAPK/ERK kinase 2 MAPKK 2 |
| Molecular Weight(MW) | 44 kDa |
| Tissue Specificity | This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394. |
| Entrez Gene | 5605 |
|---|---|
| Protein Accession | NP_109587.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).
Application
Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
