Application
Western blot analysis in U937 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of MAP2K2 using MAP2K2 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).Application
Antibody is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.Application
Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody . U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidinApplication
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MEK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.1) Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003). |
| Immunogen | This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the Central region of human MEK2 (MAP2K2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IF,IHC-P,WB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K2 |
|---|---|
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 2 MAP kinase kinase 2 MAPKK 2 ERK activator kinase 2 MAPK/ERK kinase 2 MEK 2 MAP2K2 MEK2 MKK2 PRKMK2 |
| Molecular Weight(MW) | 44 kDa |
Application
Western blot analysis in U937 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of MAP2K2 using MAP2K2 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).
Application
Antibody is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.
Application
Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody . U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
