Application
Western blot analysis in human normal Uterus tissue lysates (35ug/lane).Application
MEKK4 Antibody (Center C1081) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MEKK4 Antibody (Center C1081) for immunohistochemistry.| Product Name | MEKK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.1) Grucza, R.A., et al. Addict Biol 15(3):346-357(2010) Whitmarsh, A.J., et al. Oncogene 26(22):3172-3184(2007) Aissouni, Y., et al. Biochem. Biophys. Res. Commun. 338(2):808-814(2005) Abell, A.N., et al. J. Biol. Chem. 280(43):35793-35796(2005) Derbyshire, Z.E., et al. Mol. Cell. Biochem. 271 (1-2), 77-90 (2005) : |
| Immunogen | This MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1067-1097 amino acids from the Central region of human MEKK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K4 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase 4 MAP three kinase 1 MAPK/ERK kinase kinase 4 MEK kinase 4 MEKK 4 MAP3K4 KIAA0213 MAPKKK4 MEKK4 MTK1 |
| Molecular Weight(MW) | 182 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in human normal Uterus tissue lysates (35ug/lane).
Application
MEKK4 Antibody (Center C1081) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MEKK4 Antibody (Center C1081) for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
