Application
Antibody is used in Western blot to detect MEKK6 in NIH3T3 cell lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MEKK6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK6 activates the JNK, but not ERK or p38 kinase pathways.1) Wang, X.S., et al., Biochem. Biophys. Res. Commun. 253(1):33-37 (1998). |
| Immunogen | This MEKK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1028-1058 amino acids from the C-terminal region of human MEKK6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MAP3K6 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase 6 Apoptosis signal-regulating kinase 2 MAP3K6 ASK2 MAPKKK6 MEKK6 |
| Molecular Weight(MW) | 143 kDa |
Application
Antibody is used in Western blot to detect MEKK6 in NIH3T3 cell lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
