Application
Antibody is used in Western blot to detect MKEL in HEPG2 tissue lysateApplication
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MELK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MELK Contains 1 protein kinase domain that Belongs to the Ser/Thr protein kinase family. It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may also be involved in the inhibition of spliceosome assembly during mitosis.1) Davezac, N., et al., Oncogene 21(50):7630-7641 (2002). |
| Immunogen | This MELK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525-554 amino acids from the C-terminal region of human MELK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MELK |
|---|---|
| Alternative Names | Maternal embryonic leucine zipper kinase hMELK Protein kinase Eg3 pEg3 kinase Protein kinase PK38 hPK38 Tyrosine-protein kinase MELK MELK KIAA0175 |
| Molecular Weight(MW) | 75 kDa |
Application
Antibody is used in Western blot to detect MKEL in HEPG2 tissue lysate
Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
