Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MER/SKY (phospho Tyr749/681) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).1) Graham D.K., Cell Growth Differ. 5:647-657(1994). |
| Immunogen | MER/SKY (phospho Tyr749/681) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 749/681 (K-I-Y (p)-S-G) derived from Human MER/SKY . |
| Modification | p-Tyr749/681 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MERTK, TYRO3 |
|---|---|
| Alternative Names | C-mer MERK MERTK |
| Molecular Weight(MW) | 97 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681. |
| Entrez Gene | 10461/7301 |
|---|---|
| Protein Accession | NP_006334.2 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
