Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-MARS2 Antibody (AP7841b) in mouse testis tissue lysates (35ug/lane).| Product Name | Methionyl tRNA Synthetase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet AMP + diphosphate + L-methionyl-tRNAMet The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.1) Spencer,A.C., Biochemistry 43 (30), 9743-9754 (2004) |
| Immunogen | This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MARS2 |
|---|---|
| Alternative Names | Methionine--tRNA ligase mitochondrial Methionyl-tRNA synthetase 2 Mitochondrial methionyl-tRNA synthetase MtMetRS MARS2 |
| Molecular Weight(MW) | 67 kDa |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-MARS2 Antibody (AP7841b) in mouse testis tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
