METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.1) Bujnicki, J.M., et al., (2002) J. Mol. Evol. 55 (4), 431-444.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human METTL3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
METTL3 antibody can be used for detection of METTL3 by ELISA at 1:312500. METTL3 antibody can be used for detection of METTL3 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Raji cells at 5.0 ug/ml.
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
METTL3 antibody can be used for detection of METTL3 by ELISA at 1:312500. METTL3 antibody can be used for detection of METTL3 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store METTL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt