Application
Antibody used in WB on Human Thymus at 5.0 ug/ml.Application
Antibody used in IHC on Human Intestine.WB
(B,C) Relative mRNA and protein expression of METTL3 in N.C. cells, OE-METTL3 cells and si-METTL3 cells to show the transfection efficiency. GAPDH served as the internal control. The results are normalized to the expression of N.C. cells.WB
(B,C) Relative mRNA and protein expression of METTL3 in N.C. cells, OE-METTL3 cells and si-METTL3 cells to show the transfection efficiency. GAPDH served as the internal control. The results are normalized to the expression of N.C. cells.WB
(E) Western blot analysis of LC3II/GAPDH ratio and Beclin1. GAPDH served as the internal control.| Product Name | METTL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.1) Bujnicki, J.M., (2002) J. Mol. Evol. 55 (4), 431-444. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human METTL3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| METTL3 antibody can be used for detection of METTL3 by ELISA at 1:312500. METTL3 antibody can be used for detection of METTL3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | METTL3 |
|---|---|
| Alternative Names | METTL3 M6A IME4 Spo8 MT-A70 |
| Molecular Weight(MW) | 64 kDa |
Application
Antibody used in WB on Human Thymus at 5.0 ug/ml.
Application
Antibody used in IHC on Human Intestine.
WB
(B,C) Relative mRNA and protein expression of METTL3 in N.C. cells, OE-METTL3 cells and si-METTL3 cells to show the transfection efficiency. GAPDH served as the internal control. The results are normalized to the expression of N.C. cells.
WB
(B,C) Relative mRNA and protein expression of METTL3 in N.C. cells, OE-METTL3 cells and si-METTL3 cells to show the transfection efficiency. GAPDH served as the internal control. The results are normalized to the expression of N.C. cells.
WB
(E) Western blot analysis of LC3II/GAPDH ratio and Beclin1. GAPDH served as the internal control.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | METTL3 antibody can be used for detection of METTL3 by ELISA at 1:312500. METTL3 antibody can be used for detection of METTL3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store METTL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
