Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-hMGAT1-R426 Pab in Y79 cell line lysate.| Product Name | MGAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT1 (UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I) is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis.1) Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995). |
| Immunogen | This MGAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MGAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MGAT1 |
|---|---|
| Alternative Names | Alpha-1 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I GNT-I GlcNAc-T I MGAT1 GGNT1 GLCT1 GLYT1 MGAT |
| Molecular Weight(MW) | 51 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-hMGAT1-R426 Pab in Y79 cell line lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
