Application
Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 μg/mL.Application
Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 μg/ml.Application
Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.| Product Name | MIB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).1) Jin Y, Blue EK, Dixon S, et al. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular level of DAPK. J. Biol. Chem. 2002; 277:46980-6. |
| Immunogen | MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MIB1 |
|---|---|
| Alternative Names | MIB1 Antibody: MIB DIP1 ZZZ6 DIP-1 LVNC7 ZZANK2 KIAA1323 E3 ubiquitin-protein ligase MIB1 DAPK-interacting protein 1 |
| Molecular Weight(MW) | Predicted: 89, 102, 111 kDa Observed: 103 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
| Tissue Specificity | MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist. |
Application
Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 μg/mL.
Application
Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 μg/ml.
Application
Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MIB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MIB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
