E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors (By similarity).1) Takeuchi T.,et al.Am. J. Pathol. 163:1395-1404(2003).
Immunogen
This MIB2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 216-249 amino acids from human MIB2.
Western Blot at 1:1000 dilution + mouse heart lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.