Application
Confocal immunofluorescent analysis of MIC1 Antibody with MDA-MBA231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | MIC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | C18orf8 is an uncharacterized protein.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This MIC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-66 amino acids from the N-terminal region of human MIC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | C18orf8 |
|---|---|
| Alternative Names | Uncharacterized protein C18orf8 Colon cancer-associated protein Mic1 Mic-1 C18orf8 MIC1 |
| Molecular Weight(MW) | 75 kDa |
Application
Confocal immunofluorescent analysis of MIC1 Antibody with MDA-MBA231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
