Application
Immunohistochemistry of MICA in human colon tissue with MICA antibody at 10 μg/mL.Application
Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 μg/mL.Application
Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | MICA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MICA Antibody: Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.1) Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol. 2006; 24:419-66. |
| Immunogen | MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MICA |
|---|---|
| Alternative Names | MICA Antibody: PERB11.1 PERB11.1 MHC class I polypeptide-related sequence A MIC-A |
| Molecular Weight(MW) | Predicted: 42 kDa Observed: 46 kDa |
| Sequence Similarities | 2) Cat. No. 1320 - Human Colon Tissue Lysate |
Application
Immunohistochemistry of MICA in human colon tissue with MICA antibody at 10 μg/mL.
Application
Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 μg/mL.
Application
Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MICA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MICA Antibody is supplied in PBS containing 0.02% sodium azide. |
