Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | MICA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MICA is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.1) Boissel, N., (2006) J. Immunol. 176 (8), 5108-5116. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MICA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MICA antibody can be used for detection of MICA by ELISA at 1:312500. MICA antibody can be used for detection of MICA by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MICA |
|---|---|
| Alternative Names | MICA DAQB-48K1.7 MGC111087 PERB11.1 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MICA antibody can be used for detection of MICA by ELISA at 1:312500. MICA antibody can be used for detection of MICA by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MICA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
