Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Product Name | MID1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined.1) Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009) |
| Immunogen | This MID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the C-terminal region of human MID1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MID1 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase Midline-1 632- Midin Putative transcription factor XPRF RING finger protein 59 RING finger protein Midline-1 Tripartite motif-containing protein 18 MID1 FXY RNF59 TRIM18 XPRF |
| Molecular Weight(MW) | 75 kDa |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
