| Product Name | MIG Antibody [1.1_2D3-2H3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MIG, a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. |
| Immunogen | The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hMIG as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Sandwich: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL9 |
|---|---|
| Alternative Names | monokine induced by interferon-gamma CMK MIG SCYB9 C-X-C motif chemokine 9 Gamma-interferon-induced monokine HuMIG Humig crg-10 |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human MIG when used with our biotinylated antigen affinity purified anti-human MIG (XP-5231Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Sandwich: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MIG lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
