Application
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.Application
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Mimitin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.1) Tsuneoka M, Teye K, Arima M, et al. A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma. J. Biol. Chem.2005; 280:19977-85. |
| Immunogen | Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin. The immunogen is located within the last 50 amino acids of Mimitin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFAF2 |
|---|---|
| Alternative Names | Mimitin Antibody: MMTN B17.2L mimitin NDUFA12L Mimitin mitochondrial Myc-induced mitochondrial protein MMTN |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
Application
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.
Application
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Mimitin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Mimitin Antibody is supplied in PBS containing 0.02% sodium azide. |
