Application
To detect Rat MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat MIP-1-alpha (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1-alpha.| Product Name | MIP-1a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MIP-1alpha (Macrophage Inflammatory Protein -1 alpha). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP-1α (100 ng/mL), a concentration of 8.0 - 10.0 μg/mL of this antibody is required. ELISA: To detect Rat MIP-1α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MIP-1α. Sandwich: To detect Rat MIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MIP-1α (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1α. Western Blot: To detect Rat MIP-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ccl3 |
|---|---|
| Alternative Names | Scya3 MIP-1a Mip1a Scya3 C-C motif chemokine 3 Macrophage inflammatory protein 1-alpha MIP-1-alpha |
Application
To detect Rat MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat MIP-1-alpha (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1-alpha.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP-1α (100 ng/mL), a concentration of 8.0 - 10.0 μg/mL of this antibody is required. ELISA: To detect Rat MIP-1α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MIP-1α. Sandwich: To detect Rat MIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MIP-1α (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1α. Western Blot: To detect Rat MIP-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Form | Liquid |
|---|---|
| Storage Instructions | MIP-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
