Application
Western blot analysis of extracts from K562 cells treated with Heatshock using Phospho-MKP-1/2 (Ser296/318) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | MKP-1/2 (phospho Ser296/318) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3CH134, CL100, Dual specificity protein phosphatase 1, DUS1, DUSP1 |
| Product description | Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.1) Lin YW, Yang JL (2006) J Biol Chem 281, 915-26 |
| Immunogen | MKP-1/2 (phospho Ser296/318) antibody was raised against a peptide sequence around phosphorylation site of serine 296/318 (I-I-S (p)-P-N) derived from Human MKP-1/2 . |
| Modification | p-Ser296/318 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DUSP1, DUSP4 |
|---|---|
| Alternative Names | 3CH134 CL100 Dual specificity protein phosphatase 1 DUS1 DUSP1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | The antibody detects endogenous level of MKP-1/2 only when phosphorylated at serine 296/318. |
| Entrez Gene | 1843/1846 |
|---|---|
| Protein Accession | NP_004408.1/ NP_001385.1 |
Application
Western blot analysis of extracts from K562 cells treated with Heatshock using Phospho-MKP-1/2 (Ser296/318) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
