Application
Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.Application
Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.| Product Name | MKRN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Makorin ring finger 3, CPPB2, D15S9, RNF63, ZFP127, ZNF127 |
| Product description | The Makorin ring finger 3 (MKRN3) protein contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. The MKRN3 gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome (1), but a deletion of the gene does not (2). A deficiency of MKRN3 has been shown to cause central precocious puberty in humans (3).1) Jong MT, Gray TA, Ji Y, et al. A novel imprinted gene, encoding a RING zinc-finger protein, a overlapping antisense transcript in the Prader-Willi syndrome critical region. Hum. Mol. Genet. 1999; 8:783-93. |
| Immunogen | MKRN3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MKRN3. The immunogen is located within amino acids 400 - 450 of MKRN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MKRN3 |
|---|---|
| Alternative Names | Makorin ring finger 3 CPPB2 D15S9 RNF63 ZFP127 ZNF127 |
| Molecular Weight(MW) | Predicted: 55 kDa Observed: 55 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family. |
Application
Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.
Application
Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MKRN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MKRN3 antibody is supplied in PBS containing 0.02% sodium azide. |
